Oligosaccharides of human milk: I. Isolation and characterization
A Kobata, V Ginsburg, M Tsuda - Archives of Biochemistry and Biophysics, 1969 - Elsevier
A Kobata, V Ginsburg, M Tsuda
Archives of Biochemistry and Biophysics, 1969•ElsevierA method involving Sephadex gel filtration and paper chromatography is described for the
separation and determination of the known oligosaccharides in individual samples of human
milk. Using this method, it was found that the four oligosaccharides which contain O-α-l-
fucosyl-(1→∗ 2)-O-β-d-galactosyl groupings occur in milk from donors with blood type Le
(b+) but not in milk from donors with blood type Le (a+).
separation and determination of the known oligosaccharides in individual samples of human
milk. Using this method, it was found that the four oligosaccharides which contain O-α-l-
fucosyl-(1→∗ 2)-O-β-d-galactosyl groupings occur in milk from donors with blood type Le
(b+) but not in milk from donors with blood type Le (a+).
Abstract
A method involving Sephadex gel filtration and paper chromatography is described for the separation and determination of the known oligosaccharides in individual samples of human milk. Using this method, it was found that the four oligosaccharides which contain O-α-l-fucosyl-(1 →∗2)-O-β-d-galactosyl groupings occur in milk from donors with blood type Le(b+) but not in milk from donors with blood type Le(a+).
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