Regulatory proteins for the activated third and fourth components of complement (C3b and C4b) in mice. I. Isolation and characterization of factor H: the serum cofactor …
T Kinoshita, V Nussenzweig - Journal of immunological methods, 1984 - Elsevier
Factor H, purified from mouse EDTA-plasma using a 4-step procedure, consists of a single
polypeptide chain of M r 150,000 on SDS-PAGE. Mouse H (H mo) was required for the
cleavage of fluid-phase mouse C3b by mouse I (I mo). The final product of degradation of
fluid-phase mouse C3b was iC3b, consisting of fragments of the α′-chain (α′-70, α′-43)
linked by disulfide bonds to an intact β-chain. I mo alone was capable of cleavage of
membrane-bound mouse C3b and of generating iC3b. The addition of H mo nevertheless …
polypeptide chain of M r 150,000 on SDS-PAGE. Mouse H (H mo) was required for the
cleavage of fluid-phase mouse C3b by mouse I (I mo). The final product of degradation of
fluid-phase mouse C3b was iC3b, consisting of fragments of the α′-chain (α′-70, α′-43)
linked by disulfide bonds to an intact β-chain. I mo alone was capable of cleavage of
membrane-bound mouse C3b and of generating iC3b. The addition of H mo nevertheless …