Antibodies to purified bee venom proteins and peptides: I. Development of a highly specific RAST for bee venom antigens and its application to bee sting allergy
DM Kemeny, MG Harries, LJF Youlten… - Journal of allergy and …, 1983 - Elsevier
IgE antibodies to purified proteins and peptides from honeybee venom have been measured
by the RAST. Trace amounts (< 0.1%) of the major venom protein phospholipase A 2 (PLA
2) grossly distorted the measurement of IgE antibody to the other venom proteins, acid
phosphatase (Acid P) and hyaluronidase (HYAL), and overemphasized their importance.
Reduction of antigen coupled to the cellulose paper discs, which were used in the assay,
diluted out the contaminating PLA 2 without apparent loss in sensitivity. The reduction of disc …
by the RAST. Trace amounts (< 0.1%) of the major venom protein phospholipase A 2 (PLA
2) grossly distorted the measurement of IgE antibody to the other venom proteins, acid
phosphatase (Acid P) and hyaluronidase (HYAL), and overemphasized their importance.
Reduction of antigen coupled to the cellulose paper discs, which were used in the assay,
diluted out the contaminating PLA 2 without apparent loss in sensitivity. The reduction of disc …
