Contribution of vraSR and graSR Point Mutations to Vancomycin Resistance in Vancomycin-Intermediate Staphylococcus aureus
We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus
(VSSA) strain, Mu50Ω, a strain related to vancomycin-intermediate S. aureus (VISA) strain
Mu50. Using a combination of Mu50Ω whole-genome sequencing and genome
engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to
VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Ω.
(VSSA) strain, Mu50Ω, a strain related to vancomycin-intermediate S. aureus (VISA) strain
Mu50. Using a combination of Mu50Ω whole-genome sequencing and genome
engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to
VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Ω.
Abstract
We describe here the genetic analysis of a vancomycin-susceptible Staphylococcus aureus (VSSA) strain, Mu50Ω, a strain related to vancomycin-intermediate S. aureus (VISA) strain Mu50. Using a combination of Mu50Ω whole-genome sequencing and genome engineering, we observed a stepwise evolution of vancomycin resistance from VSSA to VISA after the mutated vraS and graR genes of Mu50 were engineered into Mu50Ω.
American Society for Microbiology