A reprogrammable mouse strain from gene-targeted embryonic stem cells
M Stadtfeld, N Maherali, M Borkent, K Hochedlinger - Nature methods, 2010 - nature.com
M Stadtfeld, N Maherali, M Borkent, K Hochedlinger
Nature methods, 2010•nature.comThe derivation of induced pluripotent stem cells (iPSCs) usually involves the viral
introduction of reprogramming factors into somatic cells. Here we used gene targeting to
generate a mouse strain with a single copy of an inducible, polycistronic reprogramming
cassette, allowing for the induction of pluripotency in various somatic cell types. As
these'reprogrammable mice'can be easily bred, they are a useful tool to study the
mechanisms underlying cellular reprogramming.
introduction of reprogramming factors into somatic cells. Here we used gene targeting to
generate a mouse strain with a single copy of an inducible, polycistronic reprogramming
cassette, allowing for the induction of pluripotency in various somatic cell types. As
these'reprogrammable mice'can be easily bred, they are a useful tool to study the
mechanisms underlying cellular reprogramming.
Abstract
The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.
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