PIAS1 and PIASxα function as SUMO-E3 ligases toward androgen receptor and repress androgen receptor-dependent transcription
T Nishida, H Yasuda - Journal of Biological Chemistry, 2002 - ASBMB
The androgen receptor (AR) has been shown to be modified by SUMO-1, a ubiquitin-like
protein. Recently we showed that PIAS family proteins function as SUMO-E3 ligases. Here
we provide evidence that PIAS1 and PIASxα act as specific SUMO-E3 ligases for the AR.
PIAS1 and PIASxα but not PIAS3 or PIASxβ enhanced the sumoylation of AR in intact cells
and in vitro. PIAS1 and PIASxα bound Ubc9, the E2 enzyme for SUMO-1, in a RING finger-
like domain-dependent manner. Consistent with previous studies (Kahyo, T., Nishida, T …
protein. Recently we showed that PIAS family proteins function as SUMO-E3 ligases. Here
we provide evidence that PIAS1 and PIASxα act as specific SUMO-E3 ligases for the AR.
PIAS1 and PIASxα but not PIAS3 or PIASxβ enhanced the sumoylation of AR in intact cells
and in vitro. PIAS1 and PIASxα bound Ubc9, the E2 enzyme for SUMO-1, in a RING finger-
like domain-dependent manner. Consistent with previous studies (Kahyo, T., Nishida, T …
