[HTML][HTML] Large adipocytes function as antigen-presenting cells to activate CD4+ T cells via upregulating MHCII in obesity
International journal of obesity, 2016•nature.com
Methods: Adipocytes were sorted by fluorescence-activated cell sorting (FACS) according to
the cell size from MAO mice. The activation of MHCII, T cells and related signaling molecules
were examined by FACS, ELISA and western blotting. 3T3-L1 cell line and primary
adipocytes were used to examine the effect of free fatty acids (FFA) on adipocytes
enlargement and MHCII expression. Results: MAO mice had a significant increase in
adipocytes size and FFA concentration. The large adipocytes from both obese and non …
the cell size from MAO mice. The activation of MHCII, T cells and related signaling molecules
were examined by FACS, ELISA and western blotting. 3T3-L1 cell line and primary
adipocytes were used to examine the effect of free fatty acids (FFA) on adipocytes
enlargement and MHCII expression. Results: MAO mice had a significant increase in
adipocytes size and FFA concentration. The large adipocytes from both obese and non …
Methods:
Adipocytes were sorted by fluorescence-activated cell sorting (FACS) according to the cell size from MAO mice. The activation of MHCII, T cells and related signaling molecules were examined by FACS, ELISA and western blotting. 3T3-L1 cell line and primary adipocytes were used to examine the effect of free fatty acids (FFA) on adipocytes enlargement and MHCII expression.
Results:
MAO mice had a significant increase in adipocytes size and FFA concentration. The large adipocytes from both obese and non-obese mice expressed higher levels of MHCII than small adipocytes. Importantly, large adipocytes from obese mice stimulated CD4+ T cells to secrete more interferon (IFN)-γ. Furthermore, the activation of the JNK-STAT1 pathway was involved in upregulation of MHCII in large adipocytes. In vitro FFA treatment promoted adipocyte hypertrophy and expression of MHCII-associated genes.
Conclusions:
This study demonstrates that large adipocytes highly express MHCII and function as APC to stimulate IFN-γ-expressing CD4+ T cells, in which FFA may have important roles before IFN-γ elevated. These findings suggest that adipocyte hypertrophy, rather than overall obesity, is the major contributor to adipose tissue inflammation and insulin resistance.
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