Compromised B cell responses to influenza vaccination in HIV-infected individuals
A Malaspina, S Moir, SM Orsega… - The Journal of …, 2005 - academic.oup.com
A Malaspina, S Moir, SM Orsega, J Vasquez, NJ Miller, ET Donoghue, S Kottilil, M Gezmu…
The Journal of infectious diseases, 2005•academic.oup.comBackground Yearly influenza vaccination, although recommended for human
immunodeficiency virus (HIV)–infected individuals, has not received thorough evaluation in
the era of antiretroviral therapy. We assessed the impact of HIV disease on B cell responses
to influenza vaccination Methods Sixty-four HIV-infected and 17 HIV-negative individuals
received the 2003–2004 trivalent inactivated influenza vaccine. Frequencies of influenza-
specific antibody-secreting cells (ASCs) were measured by enzyme-linked immunospot …
immunodeficiency virus (HIV)–infected individuals, has not received thorough evaluation in
the era of antiretroviral therapy. We assessed the impact of HIV disease on B cell responses
to influenza vaccination Methods Sixty-four HIV-infected and 17 HIV-negative individuals
received the 2003–2004 trivalent inactivated influenza vaccine. Frequencies of influenza-
specific antibody-secreting cells (ASCs) were measured by enzyme-linked immunospot …
Abstract
BackgroundYearly influenza vaccination, although recommended for human immunodeficiency virus (HIV)–infected individuals, has not received thorough evaluation in the era of antiretroviral therapy. We assessed the impact of HIV disease on B cell responses to influenza vaccination
MethodsSixty-four HIV-infected and 17 HIV-negative individuals received the 2003–2004 trivalent inactivated influenza vaccine. Frequencies of influenza-specific antibody-secreting cells (ASCs) were measured by enzyme-linked immunospot (ELISPOT) assay, and antibody responses were measured by hemagglutination-inhibition (HI) assay. Memory responses to influenza were measured by ELISPOT assay after polyclonal activation of B cells in vitro
ResultsPrevaccination HI titers were significantly higher in HIV-negative than in HIV-infected individuals. Peak HI titers and influenza-specific ASC frequencies were directly correlated with CD4+ T cell counts in HIV-infected individuals. Influenza-specific memory B cell responses were significantly lower in HIV-infected than in HIV-negative individuals and were directly correlated with CD4+ T cell counts
ConclusionsHIV infection is associated with a weak antibody response to influenza vaccination that is compounded by a poor memory B cell response. CD4+ T cell count is a critical determinant of responsiveness to influenza vaccination, and the contribution of plasma HIV RNA level is suggestive and warrants further investigation
Oxford University Press