Calcium-phosphate-mediated transfection of eukaryotic cells with plasmid DNAs
J Sambrook, DW Russell - Cold Spring Harbor Protocols, 2006 - cshprotocols.cshlp.org
J Sambrook, DW Russell
Cold Spring Harbor Protocols, 2006•cshprotocols.cshlp.orgMETHOD 1. Twenty-four hours before transfection, harvest exponentially growing cells by
trypsinization and replate them at a density of 1 x 10 5 to 4 x 10 5 cells/cm 2 in 60-mm tissue
culture dishes or 12-well plates in the appropriate complete medium. Incubate the cultures
for 20-24 hours at 37 C in a humidified incubator with an atmosphere of 5-7% CO 2. Change
the medium 1 hour before transfection.
trypsinization and replate them at a density of 1 x 10 5 to 4 x 10 5 cells/cm 2 in 60-mm tissue
culture dishes or 12-well plates in the appropriate complete medium. Incubate the cultures
for 20-24 hours at 37 C in a humidified incubator with an atmosphere of 5-7% CO 2. Change
the medium 1 hour before transfection.
METHOD
1. Twenty-four hours before transfection, harvest exponentially growing cells by trypsinization and replate them at a density of 1 x 10 5 to 4 x 10 5 cells/cm 2 in 60-mm tissue culture dishes or 12-well plates in the appropriate complete medium. Incubate the cultures for 20-24 hours at 37 C in a humidified incubator with an atmosphere of 5-7% CO 2. Change the medium 1 hour before transfection.
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