[HTML][HTML] The C terminus of cardiac troponin I is essential for full inhibitory activity and Ca2+ sensitivity of rat myofibrils
HM Rarick, XH Tu, RJ Solaro, AF Martin - Journal of Biological Chemistry, 1997 - ASBMB
Although the C terminus of troponin I is known to be important in myofilament Ca 2+
regulation in skeletal muscle, the regulatory function of this region of cardiac troponin I (cTnI)
has not been defined. To address this question, the following recombinant proteins were
expressed in Escherichia coli and purified: mouse wild-type cTnI (WT cTnI; 211 residues),
cTnI-(1–199)(missing 12 residues), cTnI-(1–188)(missing 23 residues), and cTnI-(1–
151)(missing 60 residues). The inhibitory activity of cTnI and the mutants was tested in …
regulation in skeletal muscle, the regulatory function of this region of cardiac troponin I (cTnI)
has not been defined. To address this question, the following recombinant proteins were
expressed in Escherichia coli and purified: mouse wild-type cTnI (WT cTnI; 211 residues),
cTnI-(1–199)(missing 12 residues), cTnI-(1–188)(missing 23 residues), and cTnI-(1–
151)(missing 60 residues). The inhibitory activity of cTnI and the mutants was tested in …