Correlative light and electron microscopy (CLEM) is used when one needs to combine both
imaging modalities on the same sample. When working on living small model organisms,
such as Caenorhabditis elegans, specific CLEM protocols are required to acquire high-
resolution light microscopic images of a region of interest and thereafter to relocate and
study the same object at the ultrastructural level using a transmission electron microscope.
In this chapter, we describe how to process living specimens from the confocal microscope …